Novaplex™ SARS-CoV-2 Variants Assays*


The Novaplex™ SARS-CoV-2 Variant Assays* are real-time reverse transcriptase polymerase chain reaction (RT-PCR) tests intended for the qualitative detection and differentiation of SARS-CoV-2 viral nucleic acids and variants.

They are multiplex real-time PCR assays designed to detect and differentiate mutations consistent with variants such as Delta or Omicron, in a single tube.

 The assays are applied on Seegene’s integrated All-in-One automated platform which enables high-throughput testing.

*For Research Use Only. Not for use in Diagnostic Procedures.


Novaplex Variants Assays*

Ordering Information

Mutation Targets
Novaplex™ SARS-CoV-2 Master Assay
69/70del, E484K, N501Y, Y144del, P681H
Novaplex™ SARS-CoV-2 Variants I Assay
69/70del, E484K, N501Y
Novaplex™ SARS-CoV-2 Variants II Assay
W152C, K417N, K417T, L452R
Novaplex™ SARS-CoV-2 P681R Assay
Novaplex™ SARS-CoV-2 Variants IV Assay
L452R, P681R, K417N
Novaplex™ SARS-CoV-2 Variants V Assay
L452Q, F490S, P681R, L452R
Novaplex™ SARS-CoV-2 Variants VI Assay
L452Q, F490S, R346K, D950N
Novaplex™ SARS-CoV-2 Variants VII Assay
69/70del, E484A, N501Y, RdRP

Features & Benefits

MULTIPLEX, REAL-TIME PCR – Detection and differentiation of essential pathogens causing infections in a single reaction
Delivers results faster at a lower cost and more accurately
Comparison of the fast track diagnostics respiratory 21 and Seegene Allplex multiplex polymerase chain reaction assays for the detection of respiratory viruses
Consistent supply
Seegene delivered more than 100 million tests in 2020
DUAL WHOLE PROCESS CONTROLS – endogenous and exogenous internal controls
Whole process validation from sample collection to extraction and PCR
SHORT TAT – (4 hours) from extraction to the final results
Delivers results faster to your customers for a faster treatment implementation
MuDT™ – Double up multiplexing capacity in any real-time PCR instrument
Enable any existing Real-time PCR instrument to be a more powerful system by doubling up its capability for multiplexing without upgrading the hardware.
Single-channel multiplexing without melting curve analysis in real-time PCR
DPO™ PRIMERS – Eliminate non-specific amplification compared to conventional primers that do not eliminate nonspecific amplification
Delivers more accurate results for better pathogen detection
Dual priming oligonucleotide system for the multiplex detection of respiratory viruses and SNP genotyping of CYP2C19 gene
POWERFUL PERFORMANCE – PCR with high sensitivity and specificity
Delivers accurate results for better patient care and peace of mind
USER-FRIENDLY WORKFLOW – Convenient workflow using Seegene’s automated one platform
Implement Seegene’s automated solution in your laboratory for faster results with minimum training
AUTOMATIC DATA ANALYZER – Automated data interpretation and LIS interlocking with Seegene Viewer
Easily retrieves results and eliminates interpretation and clerical errors
UDG SYSTEM – Utilization of the UDG system to prevent carryover contamination
Prevents carryover contamination and false-positive results found in PCR for more accurate results
A Novel Multiplex qRT-PCR Assay to Detect SARS-CoV-2 Infection: High Sensitivity and Increased Testing Capacity


Automated data analysis for multiplex real-time PCR

The Seegene Viewer is designed to enable users to simply access automated data analysis for Seegene’s high multiplex real-time PCR assays. The software allows identification and differentiation for both Ct value of multiple targets in a single channel as well as melting curve analysis.

Nucleic Acid Extraction

The assay was validated with the extraction options listed below.

Extraction Kit
Reaction No. / Catalog No.
Seegene STARlet (65415-03)
STARMag 96 X 4 Universal Cartridge Kit
384 reactions (744300.4.UC384)
Seegene STARlet (65415-03)
STARMag 96 X 4 Viral DNA/RNA 200 C Kit
384 reactions (EX00013C)
Seegene NIMBUS (67930-03)
STARMag 96 X 4 Universal Cartridge Kit
384 reactions (744300.4.UC384)
Seegene NIMBUS (67930-03)
STARMag 96 X 4 Viral DNA/RNA 200 C Kit
384 reactions (EX00013C)

Amplification and Detection

CFX96 Touch

Seegene extraction reagents/instrumentation can be purchased through Seegene Technologies (CA, US).


Research Links


The Novaplex™ SARS-CoV-2 Variants Assays* are for Research Use Only.