Novaplex™ SARS-CoV-2 Variants Assays*

Overview

The Novaplex™ SARS-CoV-2 Variant Assays* are real-time reverse transcriptase polymerase chain reaction (RT-PCR) tests intended for the qualitative detection and differentiation of SARS-CoV-2 viral nucleic acids and variants.

They are multiplex real-time PCR assays designed to detect and differentiate mutations consistent with variants such as Delta or Omicron, in a single tube.

 The assays are applied on Seegene’s integrated All-in-One automated platform which enables high-throughput testing.

*For Research Use Only

Targets

Endogenous Internal Control

Ordering Information

Novaplex™ SARS-CoV-2 Master Assay

Novaplex™ SARS-CoV-2 Variants I Assay         

Novaplex™ SARS-CoV-2 Variants II Assay        

 

Novaplex™ SARS-CoV-2 P681R Assay  

Novaplex™ SARS-CoV-2 Variants IV Assay

Novaplex™ SARS-CoV-2 Variants V Assay

Features & Benefits

Feature
Benefit
Proof
MULTIPLEX, REAL-TIME PCR – Detection and differentiation of essential pathogens causing infections in a single reaction
Delivers results faster at a lower cost and more accurately
Comparison of the fast track diagnostics respiratory 21 and Seegene Allplex multiplex polymerase chain reaction assays for the detection of respiratory viruses
MANUFACTURABILITY
Consistent supply
Seegene delivered more than 100 million tests in 2020
DUAL WHOLE PROCESS CONTROLS – endogenous and exogenous internal controls
Whole process validation from sample collection to extraction and PCR
SHORT TAT – (4 hours) from extraction to the final results
Delivers results faster to your customers for a faster treatment implementation
MuDT™ – Double up multiplexing capacity in any real-time PCR instrument
Enable any existing Real-time PCR instrument to be a more powerful system by doubling up its capability for multiplexing without upgrading the hardware.
Single-channel multiplexing without melting curve analysis in real-time PCR
DPO™ PRIMERS – Eliminate non-specific amplification compared to conventional primers that do not eliminate nonspecific amplification
Delivers more accurate results for better pathogen detection
Dual priming oligonucleotide system for the multiplex detection of respiratory viruses and SNP genotyping of CYP2C19 gene
POWERFUL PERFORMANCE – PCR with high sensitivity and specificity
Delivers accurate results for better patient care and peace of mind
USER-FRIENDLY WORKFLOW – Convenient workflow using Seegene’s automated one platform
Implement Seegene’s automated solution in your laboratory for faster results with minimum training
AUTOMATIC DATA ANALYZER – Automated data interpretation and LIS interlocking with Seegene Viewer
Easily retrieves results and eliminates interpretation and clerical errors
UDG SYSTEM – Utilization of the UDG system to prevent carryover contamination
Prevents carryover contamination and false-positive results found in PCR for more accurate results
ENDOGENOUS INTERNAL CONTROL
A Novel Multiplex qRT-PCR Assay to Detect SARS-CoV-2 Infection: High Sensitivity and Increased Testing Capacity

Results

Automated data analysis for multiplex real-time PCR

The Seegene Viewer is designed to enable users to simply access automated data analysis for Seegene’s high multiplex real-time PCR assays. The software allows identification and differentiation for both Ct value of multiple targets in a single channel as well as melting curve analysis.

Nucleic Acid Extraction

The assay was validated with the extraction options listed below.

Manufacturer
Instrument
Extraction Kit
Reaction No. / Catalog No.
Seegene
Seegene STARlet (65415-03)
STARMag 96 X 4 Universal Cartridge Kit
384 reactions (744300.4.UC384)
Seegene
Seegene STARlet (65415-03)
STARMag 96 X 4 Viral DNA/RNA 200 C Kit
384 reactions (EX00013C)
Seegene
Seegene NIMBUS (67930-03)
STARMag 96 X 4 Universal Cartridge Kit
384 reactions (744300.4.UC384)
Seegene
Seegene NIMBUS (67930-03)
STARMag 96 X 4 Viral DNA/RNA 200 C Kit
384 reactions (EX00013C)

Amplification and Detection

Manufacturer
Instrument
Bio-Rad
CFX96 Touch

Seegene extraction reagents/instrumentation can be purchased through Seegene Technologies (CA, US).

Note

The Novaplex™ SARS-CoV-2 Variants Assays* are for Research Use Only.