Overview
The Novaplex™ SARS-CoV-2/FluA/FluB/RSV Assay* is a multiplex real-time PCR assay that enables simultaneous amplification and differentiation of target nucleic acids of S gene, RdRP gene, and N gene of SARS-CoV-2, Influenza A virus (Flu A), Influenza B virus (Flu B) and Human respiratory syncytial virus (RSV) with two Internal Controls (Endogenous IC and Exogenous IC).
To perform the multiplex target amplification and detection with superior accuracy in a single reaction, this assay kit employs Seegene’s innovative proprietary DPO™, TOCE™ and MuDT™ technologies. The presence of specific gene sequences in the reaction is reported as a Ct value through Seegene Viewer analysis software.
* For Research Use Only
Analytes
- Influenza A virus (Flu A)
- Influenza B virus (Flu B)
- Respiratory syncytial virus (RSV)
- SARS-CoV-2 (N gene)
- SARS-CoV-2 (RdRP gene)
- SARS-CoV-2 (S gene)
- Endogenous Internal Control
- Exogenous Internal Control
Samples
- Sputum
- Nasopharyngeal swab
- Nasopharyngeal aspirate
- Bronchoalveolar lavage
- Throat swab
- Saliva
Ordering Information
Product
Novaplex™ SARS-CoV-2/FluA/FluB/RSV Assay
Cat No. / Size
R-RV10259W / 100 rxns
Features & Benefits
Results
Automated data analysis for multiplex real-time PCR
Seegene Viewer is designed to enable users to simply access to automated data analysis for Seegene’s high multiplex real-time PCR assays. The software allows identification and differentiation for both Ct value of multiple targets in a single channel as well as melting curve analysis.
The figure represents a positive result for S gene, RdRP gene and N gene of SARS-CoV-2 in FAM, HEX, and Cal Red 610 channel and Flu A in Cal Red 610 channel, respectively.
Nucleic Acid Extraction
The assay was validated with the extraction options listed below.
Amplification and Detection
The Seegene, Hamilton, and LG Chem, extraction reagents/instrumentation can be purchased through Seegene USA.
Note
The Novaplex™ SARS-CoV-2/FluA/FluB/RSV Assay is For Research Use Only.