Overview
The Novaplex™ SARS-CoV-2/FluA/FluB/RSV Assay* is a multiplex real-time PCR assay that enables simultaneous amplification and differentiation of target nucleic acids of S gene, RdRP gene, and N gene of SARS-CoV-2, Influenza A virus (Flu A), Influenza B virus (Flu B) and Human respiratory syncytial virus (RSV) with two Internal Controls (Endogenous IC and Exogenous IC).
To perform the multiplex target amplification and detection with superior accuracy in a single reaction, this assay kit employs Seegene’s innovative proprietary DPO™, TOCE™ and MuDT™ technologies. The presence of specific gene sequences in the reaction is reported as a Ct value through Seegene Viewer analysis software.
* For Research Use Only
Analytes
- Influenza A virus (Flu A)
- Influenza B virus (Flu B)
- Respiratory syncytial virus (RSV)
- SARS-CoV-2 (N gene)
- SARS-CoV-2 (RdRP gene)
- SARS-CoV-2 (S gene)
- Endogenous Internal Control
- Exogenous Internal Control
Samples
- Sputum
- Nasopharyngeal swab
- Nasopharyngeal aspirate
- Bronchoalveolar lavage
- Throat swab
- Saliva
Ordering Information
Product
Novaplex™ SARS-CoV-2/FluA/FluB/RSV Assay
Cat No. / Size
RV10259X / 100 rxns
Features & Benefits
Feature | Benefit | Proof |
|---|---|---|
MULTIPLEX REAL-TIME PCR – Detection and differentiation of essential 7 viruses causing respiratory tract infections in a single reaction.
| Delivers results faster at a lower cost and more accurately | |
MANUFACTURABILITY | Consistent supply | Seegene has delivered more than 100 million tests in 2020 |
ENDOGENOUS INTERNAL CONTROL | Validates correct sampling for self-collected samples | |
DUAL WHOLE PROCESS CONTROLS – Endogenous and exogenous internal controls | Whole process validation from sample collection to extraction and PCR | |
SHORT TAT –
(4 hours) from extraction to the final results
| Delivers results faster to your customers for a faster treatment implementation | |
POWERFUL PERFORMANCE –
PCR with high sensitivity and specificity
| Delivers accurate results for better patient care and peace of mind | |
USER-FRIENDLY WORKFLOW –
Convenient workflow using Seegene’s automated one platform
| Implement Seegene’s solution in your laboratory faster with minimum training | |
AUTOMATIC DATA ANALYZER –
Automated data interpretation and LIS interlocking with Seegene Viewer
| Easily retrieves results and eliminates interpretation and clerical errors | |
UDG SYSTEM
Utilization of the UDG system to prevent carry-over contamination
| Prevents carryover contamination and false positive results found in PCR for more accurate results |
Results
Automated data analysis for multiplex real-time PCR
Seegene Viewer is designed to enable users to simply access to automated data analysis for Seegene’s high multiplex real-time PCR assays. The software allows identification and differentiation for both Ct value of multiple targets in a single channel as well as melting curve analysis.
The figure represents a positive result for S gene, RdRP gene and N gene of SARS-CoV-2 in FAM, HEX, and Cal Red 610 channel and Flu A in Cal Red 610 channel, respectively.
Nucleic Acid Extraction
The assay was validated with the extraction options listed below.
Manufacturer | Instrument | Extraction Kit | Reaction No. / Catalog No. |
|---|---|---|---|
Seegene | Seegene STARlet (65415-03) | STARMag 96 X 4
Universal Cartridge Kit | 384 reactions
(744300.4.UC384) |
Seegene | Seegene STARlet (65415-03) | STARMag 96 X 4 Viral DNA/RNA 200 C Kit | 384 reactions (EX00013C) |
Hamilton | Microlab STARlet IVD
(173000-075) | STARMag 96 X 4
Universal Cartridge Kit
| 384 reactions (744300.4.UC384) |
Hamilton | Microlab STARlet IVD
(173000-075)
| STARMag 96 X 4 Viral
DNA/RNA 200 C Kit
| 384 reactions
(EX00013C)
|
Seegene | Seegene NIMBUS (67930-03) | STARMag 96 X 4
Universal Cartridge Kit
| 384 reactions (744300.4.UC384) |
Seegene | Seegene NIMBUS (67930-03) | STARMag 96 X 4 Viral DNA/RNA 200 C Kit | 384 reactions (EX00013C) |
Hamilton | Microlab NIMBUS IVD
(65415-02)
| STARMag 96 X 4
Universal Cartridge Kit
| 384 reactions (744300.4.UC384) |
Hamilton | Microlab NIMBUS IVD
(65415-02)
| STARMag 96 X 4 Viral
DNA/RNA 200 C Kit
| 384 reactions
(EX00013C)
|
LG Chem | AdvanSure E3 System (YETS0001EG) | AdvanSure NA EX Kit | 96 reactions Reagent Plate
(RPE0001K01)
|
LG Chem |
AdvanSure E3 System (YETS0001EG)
|
AdvanSure NA EX Kit
| Proteinase K Tube (RPK0001K01) |
LG Chem
| AdvanSure E3 System (YETS0001EG) | AdvanSure NA EX Kit | Strip (E3 System) (YSTP0500KG) |
Roche | Roche MagNA Pure 96
(MP96)
| DNA and Viral NA Small Volume Kit | 576 extractions
(06 543 588 001)
|
Thermo Fisher Scientific | KingFisher Flex automated extraction
(5400630)
| MagMAX Viral/Pathogen
Nucleic Acid Isolation Kit
| 200 extractions (A42352) |
Amplification and Detection
Manufacturer | Instrument |
|---|---|
Bio-Rad | CFX96™ Real-time PCR System |
Bio-Rad | CFX96™ Dx System |
The Seegene, Hamilton, and LG Chem, extraction reagents/instrumentation can be purchased through Seegene Technologies (California, US).
Note
The Novaplex™ SARS-CoV-2/FluA/FluB/RSV Assay is For Research Use Only.