Novaplex™ SARS-CoV-2 / FluA / FluB / RSV Assay*

Overview

The Novaplex™ SARS-CoV-2/FluA/FluB/RSV Assay* is a multiplex real-time PCR assay that enables simultaneous amplification and differentiation of target nucleic acids of S gene, RdRP gene, and N gene of SARS-CoV-2, Influenza A virus (Flu A), Influenza B virus (Flu B) and Human respiratory syncytial virus (RSV) with two Internal Controls (Endogenous IC and Exogenous IC).

To perform the multiplex target amplification and detection with superior accuracy in a single reaction, this assay kit employs Seegene’s innovative proprietary DPO™, TOCE™ and MuDT™ technologies. The presence of specific gene sequences in the reaction is reported as a Ct value through Seegene Viewer analysis software.

* For Research Use Only

Analytes
  • Influenza A virus (Flu A)
  • Influenza B virus (Flu B)
  • Respiratory syncytial virus (RSV)
  • SARS-CoV-2 (N gene)
  • SARS-CoV-2 (RdRP gene)
  • SARS-CoV-2 (S gene)
  • Endogenous Internal Control
  • Exogenous Internal Control
Samples
  • Sputum
  • Nasopharyngeal swab
  • Nasopharyngeal aspirate
  • Bronchoalveolar lavage
  • Throat swab
  • Saliva

Ordering Information

Product

Novaplex™ SARS-CoV-2/FluA/FluB/RSV Assay     

Cat No. / Size

RV10259X / 100 rxns

Features & Benefits

Feature
Benefit
Proof
MULTIPLEX REAL-TIME PCR – Detection and differentiation of essential 7 viruses causing respiratory tract infections in a single reaction.
Delivers results faster at a lower cost and more accurately
MANUFACTURABILITY
Consistent supply
Seegene has delivered more than 100 million tests in 2020
ENDOGENOUS INTERNAL CONTROL
Validates correct sampling for self-collected samples
DUAL WHOLE PROCESS CONTROLS – Endogenous and exogenous internal controls
Whole process validation from sample collection to extraction and PCR
SHORT TAT – (4 hours) from extraction to the final results
Delivers results faster to your customers for a faster treatment implementation
POWERFUL PERFORMANCE – PCR with high sensitivity and specificity
Delivers accurate results for better patient care and peace of mind
USER-FRIENDLY WORKFLOW – Convenient workflow using Seegene’s automated one platform
Implement Seegene’s solution in your laboratory faster with minimum training
AUTOMATIC DATA ANALYZER – Automated data interpretation and LIS interlocking with Seegene Viewer
Easily retrieves results and eliminates interpretation and clerical errors
UDG SYSTEM Utilization of the UDG system to prevent carry-over contamination
Prevents carryover contamination and false positive results found in PCR for more accurate results

Results

Automated data analysis for multiplex real-time PCR

Seegene Viewer is designed to enable users to simply access to automated data analysis for Seegene’s high multiplex real-time PCR assays. The software allows identification and differentiation for both Ct value of multiple targets in a single channel as well as melting curve analysis.

The figure represents a positive result for S gene, RdRP gene and N gene of SARS-CoV-2 in FAM, HEX, and Cal Red 610 channel and Flu A in Cal Red 610 channel, respectively.

Nucleic Acid Extraction

The assay was validated with the extraction options listed below.

Manufacturer
Instrument
Extraction Kit
Reaction No. / Catalog No.
Seegene
Seegene STARlet (65415-03)
STARMag 96 X 4 Universal Cartridge Kit
384 reactions (744300.4.UC384)
Seegene
Seegene STARlet (65415-03)
STARMag 96 X 4 Viral DNA/RNA 200 C Kit
384 reactions (EX00013C)
Hamilton
Microlab STARlet IVD (173000-075)
STARMag 96 X 4 Universal Cartridge Kit
384 reactions (744300.4.UC384)
Hamilton
Microlab STARlet IVD (173000-075)
STARMag 96 X 4 Viral DNA/RNA 200 C Kit
384 reactions (EX00013C)
Seegene
Seegene NIMBUS (67930-03)
STARMag 96 X 4 Universal Cartridge Kit
384 reactions (744300.4.UC384)
Seegene
Seegene NIMBUS (67930-03)
STARMag 96 X 4 Viral DNA/RNA 200 C Kit
384 reactions (EX00013C)
Hamilton
Microlab NIMBUS IVD (65415-02)
STARMag 96 X 4 Universal Cartridge Kit
384 reactions (744300.4.UC384)
Hamilton
Microlab NIMBUS IVD (65415-02)
STARMag 96 X 4 Viral DNA/RNA 200 C Kit
384 reactions (EX00013C)
LG Chem
AdvanSure E3 System (YETS0001EG)
AdvanSure NA EX Kit
96 reactions Reagent Plate (RPE0001K01)
LG Chem
AdvanSure E3 System (YETS0001EG)
AdvanSure NA EX Kit
Proteinase K Tube (RPK0001K01)
LG Chem
AdvanSure E3 System (YETS0001EG)
AdvanSure NA EX Kit
Strip (E3 System) (YSTP0500KG)
Roche
Roche MagNA Pure 96 (MP96)
DNA and Viral NA Small Volume Kit
576 extractions (06 543 588 001)
Thermo Fisher Scientific
KingFisher Flex automated extraction (5400630)
MagMAX Viral/Pathogen Nucleic Acid Isolation Kit
200 extractions (A42352)

Amplification and Detection

Manufacturer
Instrument
Bio-Rad
CFX96™ Real-time PCR System
Bio-Rad
CFX96™ Dx System
The Seegene, Hamilton, and LG Chem, extraction reagents/instrumentation can be purchased through Seegene Technologies (California, US).

Note

The Novaplex™ SARS-CoV-2/FluA/FluB/RSV Assay is For Research Use Only.

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High Multiplex RT-PCR Testing for COVID-19 and Other Pathogens