Novaplex™ Assays*
High-throughput multiplex testing for identification of unknown pathogens
Seegene’s Novaplex assays provide more information for infections than simplex assays
The Seegene Novaplex Assay panels are Research Use Only (RUO) real-time reverse transcriptase polymerase chain reaction (RT-PCR) tests that maximize test efficiency with excellent performance.
Because Seegene Novaplex assays simultaneously test for multiple targets in reduced running time with minimal labor required. They reduce overall test cost and improve test productivity.
Full panels provide much shorter test times for multiple parasitic, viral or bacterial infections, while reducing labor and reagent/media cost and still providing the highest level of sensitivity.
Seegene’s integrated all-in-one automated platform enable high-throughput testing.
*NOTE: Novaplex assay panels are for Research Use Only.
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Technology
MuDT™
Individual Ct Values for multiple targets MUDT™ is the world’s first Real-time PCR technology that provides individual Ct values for multiple targets.
MuDT™ technology is the novel analytical real-time PCR technology, which enables us to detect multiple targets with individual Ct value in a single channel without melting curve analysis.
TOCE™
The New Paradigm for High multiplex Real-time PCR TOCE™ technology enables confirmation of multiple (five or more) target genetic detection and genetic variation.
TOCE™ Technology is very elegant oligonucleotide chemistry solution for the homogenous high multiplex real-time PCR. TOCE™ technology overcomes the current technical limitations of target-based probe real-time PCR technology and fully exploits the potential of real-time PCR in high multiplex analysis, through unique signal generation & melting temperature analysis by novel components.
DPO™
Novel Oligo platforms of super multiplex PCR.
DPO™ Technology is the innovative multiple targets amplification technology that enhances target specificity and minimizes the non-specific amplification commonly occurred in multiplex PCR. DPO™ technology redefines high multiplex PCR by enabling to detect “many targets in a single tube” and is well-suited for high multiplex real-time PCR technology.
Features and Benefits
Features | Benefits | Proof |
|---|---|---|
MULTIPLEX, REAL-TIME PCR –
Detection and differentiation of essential pathogens causing infections in a single reaction
| Delivers results faster at a lower cost and more accurately | Comparison of the fast track diagnostics respiratory 21 and Seegene Allplex multiplex polymerase chain reaction assays for the detection of respiratory viruses |
MANUFACTURABILITY | Consistent supply | Seegene has delivered hundreds of million tests |
ENDOGENOUS INTERNAL CONTROL | Validates correct sampling for self-collected samples | A Novel Multiplex qRT-PCR Assay to Detect SARS-CoV-2 Infection: High Sensitivity and Increased Testing Capacity |
SHORT TAT – (4 hours) from extraction to the final results
| Delivers results faster to your customers for a faster treatment implementation | |
POWERFUL PERFORMANCE –
PCR with high sensitivity and specificity | Delivers accurate results for better patient care and peace of mind | Performance of Anyplex™ II multiplex real-time PCR for the diagnosis of seven sexually transmitted infections: comparison with currently available methods |
USER-FRIENDLY WORKFLOW –
Convenient workflow using Seegene’s automated one platform
| Implement Seegene’s solution in your laboratory faster with minimum training | |
MuDT™ – Double up multiplexing capacity in any real-time PCR instrument
| Enable any existing Real-time PCR instrument to be more powerful system by doubling up its capability for multiplexing without upgrading the hardware | Single-channel multiplexing without melting curve analysis in real-time PCR |
DPO™ PRIMERS – Eliminate non-specific amplification compared to conventional primers that do not eliminate nonspecific amplification | Delivers more accurate results for better pathogen detection | Dual priming oligonucleotide system for the multiplex detection of respiratory viruses and SNP genotyping of CYP2C19 gene |
AUTOMATIC DATA ANALYSIS – Automated data interpretation and LIS connectivity with Seegene Viewer. | Easily retrieves results and eliminates interpretation and clerical errors | |
UDG SYSTEM – Utilization of the UDG system to prevent carry-over contamination
| Prevents carryover contamination and false positive results found in PCR for more accurate results | |
SEMI-QUANTITATIVE RESULTS OR SEMI-QUANTITATION | Patented MuDT technology enables multiple Ct values per fluorescent color channel |
Results
Automated data analysis for multiplex real-time PCR
Seegene Viewer is designed to enable users to simply access to automated data analysis for Seegene’s high multiplex real-time PCR assays. The software allows identification and differentiation for both Ct value of multiple targets in a single channel as well as melting curve analysis.
Panels
COVID-19 and Variants
Women's Health
| ANALYTES | Chlamydia trachomatis (CT) | CATALOG NUMBER |
| Neisseria gonorrhoeae (NG) | R-SD9801W | |
| Mycoplasma genitalium (MG) | ||
| Mycoplasma hominis (MH) | ||
| Trichomonas vaginalis (TV) | ||
| Ureaplasma urealyticum (UU) | ||
| Ureaplasma parvum (UP) | ||
| Internal Control (IC) |
| ANALYTES | Herpes simplex virus type 1 (HSV1) | CATALOG NUMBER |
| Herpes simplex virus type 2 (HSV2) | R-SD9802W | |
| Cytomegalovirus (CMV) | ||
| Varicella-zoster virus (VZV) | ||
| Lymphogranuloma venereum (LGV) | ||
| Treponema pallidum (TP) | ||
| Haemophilus ducreyi (HD) | ||
| Internal Control (IC) |
| ANALYTES | Candida albicans (CA) | Catalog Number |
| Candida glabrata (CG) | R-SD9803W | |
| Candida tropicalis (CTp) | ||
| Candida parapsilosis (CP) | ||
| Candida krusei (CK) | ||
| Candida lusitaniae (CL) | ||
| Candida dubliniensis (CD) | ||
| Internal Control (IC) |
| ANALYTES | Gardnerella vaginalis (Quantitative) (GV) | CATALOG NUMBER |
| Atopobium vaginae (Quantitative) (AV) | R-SD9804W | |
| Megasphaera Type 1 (Mega1) | ||
| Bacterial vaginosis–associated bacteria 2 (BVAB2) | ||
| Mobiluncus spp. (Mob) | ||
| Bacteroides fragilis (BF) | ||
| Lactobacillus spp. (Quantitative) (Lacto) | ||
| Internal Control (IC) |
| ANALYTES | Escherichia coli | CATALOG NUMBER |
| Staphylococcus aureus | R-SD9700W | |
| Enterococcus faecalis | ||
| Streptococcus agalactiae | ||
| Streptococcus pyogenes | ||
| Lactobacillus spp. | ||
| Lactobacillus reuteri/rhamnosus | ||
| Internal Control (IC) |
| ANALYTES | Mycoplasma genitalium (MG) | CATALOG NUMBER |
| A2058G | R-SD1017W | |
| A2058T | ||
| A2058C | ||
| A2059G | ||
| A2059T | ||
| A2059C | ||
| Internal Control (IC) |
| ANALYTES | Mycoplasma genitalium (MG) | CATALOG NUMBER |
| A247C | R-SD10170 | |
| G248T | ||
| G248A | ||
| G259A | ||
| G259T | ||
| G259C | ||
| A260G | ||
| Internal Control (IC) |
| ANALYTES | Human Papillomavirus 16 (HPV 16) | CATALOG NUMBER |
| Human Papillomavirus 18 (HPV 18) | R-HP7E00W | |
| Human Papillomavirus 31 (HPV 33) | ||
| Human Papillomavirus 33 (HPV 33) | ||
| Human Papillomavirus 35 (HPV 35) | ||
| Human Papillomavirus 39 (HPV 39) | ||
| Human Papillomavirus 45 (HPV 45) | ||
| Human Papillomavirus 51 (HPV 51) | ||
| Human Papillomavirus 52 (HPV 52) | ||
| Human Papillomavirus 56 (HPV 56) | ||
| Human Papillomavirus 58 (HPV 58) | ||
| Human Papillomavirus 59 (HPV 59) | ||
| Human Papillomavirus 66 (HPV 66) | ||
| Human Papillomavirus 68 (HPV 68) | ||
| Internal Control (IC) |
| ANALYTES | Human Papillomavirus 16 (HPV 16) | CATALOG NUMBER |
| Human Papillomavirus 18 (HPV 18) | R-HP7S00W | |
| Human Papillomavirus 26 (HPV 26) | ||
| Human Papillomavirus 31 (HPV 33) | ||
| Human Papillomavirus 33 (HPV 33) | ||
| Human Papillomavirus 35 (HPV 35) | ||
| Human Papillomavirus 39 (HPV 39) | 19 High-risk HPV genotypes: | |
| Human Papillomavirus 45 (HPV 45) | 16, 18, 26, 31, 33, 35, 39, 45, | |
| Human Papillomavirus 51 (HPV 51) | 51, 52, 53, 56, 58, 59, 66, 68, 69, 73, 82) | |
| Human Papillomavirus 52 (HPV 52) | 9 Low-risk HPV genotypes: | |
| Human Papillomavirus 53 (HPV 53) | 6, 11, 40, 42, 43, 44, 54, 61, 70) | |
| Human Papillomavirus 56 (HPV 56) | ||
| Human Papillomavirus 58 (HPV 58) | ||
| Human Papillomavirus 59 (HPV 59) | ||
| Human Papillomavirus 66 (HPV 66) | ||
| Human Papillomavirus 68 (HPV 68) | ||
| Human Papillomavirus 69 (HPV 69) | ||
| Human Papillomavirus 73 (HPV 73) | ||
| Human Papillomavirus 82 (HPV 82) | ||
| Human Papillomavirus 11 (HPV 11) | ||
| Human Papillomavirus 40 (HPV 40) | ||
| Human Papillomavirus 42 (HPV 42) | ||
| Human Papillomavirus 43 (HPV 43) | ||
| Human Papillomavirus 44 (HPV 44) | ||
| Human Papillomavirus 54 (HPV 54) | ||
| Human Papillomavirus 6 (HPV 6) | ||
| Human Papillomavirus 61 (HPV 61) | ||
| Human Papillomavirus 70 (HPV 70) | ||
| Internal Control (IC) |
| ANALYTES | Chlamydia trachomatis (CT) | CATALOG NUMBER |
| Neisseria gonorrhoeae (NG) | R-SD9400W | |
| Mycoplasma genitalium (MG) | ||
| Trichomonas vaginalis (TV) | ||
| Internal Control (IC) |
| ANALYTES | Escherichia coli | REFERENCE NUMBER |
| Pseudomonas aeruginosa | R-SD10228W | |
| Klebsiella pneumoniae | ||
| Klebsiella oxytoca | ||
| Klebsiella (Enterobacter) aerogenes | ||
| Proteus mirabilis | ||
| Proteus vulgaris | ||
| Enterobacter cloacae complex | ||
| Serratia marcescens | ||
| Internal Control (IC) |
| ANALYTES | Enterococcus faecalis | REFERENCE NUMBER |
| Enterococcus faecium | R-SD10240W | |
| Staphylococcus aureus | ||
| Streptococcus agalactiae | ||
| Staphylococcus epidermidis | ||
| Staphylococcus saprophyticus | ||
| Actinobaculum schaalii | ||
| Candida albicans | ||
| Candida others (glabrata, tropicalis, parapsilosis, krusei) | ||
| Internal Control (IC) |
| ANALYTES | Acinetobacter baumannii | REFERENCE NUMBER |
| Citrobacter freundii | R-SD10241W | |
| Citrobacter koseri | ||
| Morganella morganii | ||
| Providencia stuartii | ||
| Streptococcus anginosus | ||
| Aerococcus urinae | ||
| Corynebacterium urealyticum | ||
| Pantoea agglomerans | ||
| Internal Control (IC) |
Hospital Acquired Infection
| ANALYTES | [CPE] | CATALOG NUMBER |
| Klebsiella pneumoniae carbapenemase (KPC) | R-CR9700W | |
| Verona Integron–encoded Metallo-β-Lactamase (VIM) | ||
| New Delhi Metallo-beta-lactamase (NDM) | ||
| Imipenemase (IMP) | ||
| Oxacillinase-48 (OXA-48) | ||
| [VRE] | ||
| vanA | ||
| vanB | ||
| [ESBL] | ||
| CTX-M | ||
| Internal Control (IC) | ||
| MRSA | SA | |
| mecA | ||
| mecC | ||
| Internal Control (IC) |
Respiratory Syndromic
| ANALYTES | Influenza A virus (Flu A) | CATALOG NUMBER |
| Influenza B virus | R-RP9801W | |
| Respiratory syncytial virus A | ||
| Respiratory syncytial virus B | ||
| Influenza A-H1 (Flu A-H1) | ||
| Influenza A-H1pdm09 (Flu A-H1pdm09) | ||
| Influenza A-H3 (Flu A-H3) | ||
| Internal Control (IC) |
| ANALYTES | Adenovirus (AdV) | CATALOG NUMBER |
| Enterovirus (HEV) | R-RP9802W | |
| Parainfluenza virus 1 (PIV 1) | ||
| Parainfluenza virus 2 (PIV 2) | ||
| Parainfluenza virus 3 (PIV 3) | ||
| Parainfluenza virus 4 (PIV 4) | ||
| Metapneumovirus (MPV) | ||
| Internal Control (IC) |
| ANALYTES | Bocavirus 1/2/3/4 (HBoV) | CATALOG NUMBER |
| Rhinovirus (HRV) | R-RP9601W | |
| Coronavirus NL63 (NL63) | ||
| Coronavirus 229E (229E) | ||
| Coronavirus OC43 (OC43) | ||
| Internal Control (IC) |
| ANALYTES | Mycoplasma pneumoniae (MP) | CATALOG NUMBER |
| Chlamydia pneumoniae (CP) | R-PB10175W | |
| Legionella pneumophila (LP) | ||
| Haemophilus influenzae (HI) | ||
| Streptococcus pneumoniae (SP) | ||
| Bordetella pertussis (BP) | ||
| Bordetella parapertussis | ||
| Internal Control (IC) |
| ANALYTES | Adenovirus (AdV) | CATALOG NUMBER |
| Influenza A virus (Flu A) | R-RV7G01W | |
| Influenza B virus (Flu B) | ||
| Parainfluenza virus 1 (PIV 1) | ||
| Parainfluenza virus 2 (PIV 2) | ||
| Parainfluenza virus 3 (PIV 3) | ||
| Parainfluenza virus 4 (PIV 4) | ||
| Rhinovirus (HRV) | ||
| Internal Control (IC) | ||
| ———————————- | ||
| Respiratory syncytial virus A (RSV A) | ||
| Respiratory syncytial virus B (RSV B) | ||
| Bocavirus 1/2/3/4 (HBoV) | ||
| Metapneumovirus (MPV) | ||
| Coronavirus 229E (229E) | ||
| Coronavirus NL63 (NL63) | ||
| Coronavirus OC43 (OC43) | ||
| Enterovirus (HEV) | ||
| Internal Control (IC) | ||
| ANALYTES | Influenza A virus (Flu A) | CATALOG NUMBER |
| Influenza B virus (Flu B) | R-RV9750W | |
| Respiratory syncytial virus (RSV) | ||
| Metapneumovirus (MPV) | ||
| Adenovirus (AdV) | ||
| Parainfluenza virus (PIV) | ||
| Rhinovirus A/B/C (HRV) | ||
| Internal Control (IC) |
| ANALYTES | Mycoplasma pneumoniae (MP) | CATALOG NUMBER |
| Chlamydophila pneumoniae (CP) | R-RB7500W | |
| Legionella pneumophila (LP) | ||
| Bordetella pertussis (BP) | ||
| Bordetella parapertussis (BPP) | ||
| Internal Control (IC) |
Gastrointestinal
| ANALYTES | Norovirus GI (NoV-GI) | CATALOG NUMBER |
| Norovirus GII (NoV-GII) | R-GI9701W | |
| Rotavirus (RotV) | ||
| Adenovirus (AdV) | ||
| Astrovirus (AstV) | ||
| Sapovirus (SV) | ||
| Internal Control (IC) |
| ANALYTES | Campylobacter spp. (Cam) | CATALOG NUMBER |
| Clostridium difficile toxin B (CdB) | R-GI9703W | |
| Salmonella spp. (Sal) | ||
| Shigella spp./EIEC (Sh/EI) | ||
| Vibrio spp. (Vib) | ||
| Yersinia enterocolitica (Yer) | ||
| Aeromonas spp. (Aer) | ||
| Internal Control (IC) |
| ANALYTES | Hypervirulent Clostridium difficile (CD hyper) | CATALOG NUMBER |
| Escherichia coli O157 (E. coli O157) | R-GI9702W | |
| STEC (stx1/2) | ||
| EPEC (eaeA) | ||
| ETEC (lt/st) | ||
| EAEC ( aggR ) | ||
| Internal Control (IC) |
| ANALYTES | Giardia lamblia (GL) | CATALOG NUMBER |
| Entamoeba histolytica (EH) | R-GI9703W | |
| Cryptosporidium spp. (CR) | ||
| Blastocystis hominis (BH) | ||
| Dientamoeba fragilis (DF) | ||
| Cyclospora cayetanensis (CC) | ||
| Internal Control (IC) | ||
| Helminthiasis I | Microsporidiosis | |
| Strongyloidiasis | ||
| Hymenolepiasis | ||
| Ascariasis | ||
| Taeniasis | ||
| Ancylostomiasis | ||
| Trichuriasis (Trichuris Trichiura) | ||
| Enterobiasis (Enterobius vermicularis) | ||
| Ancylostomiasis (Necator Americanus) | ||
| Internal Control (IC) |
Tuberculosis
| ANALYTES | Mycobacterium tuberculosis (MTB) | CATALOG NUMBER |
| Isoniazid-resistance (7 mutations) | R-TB7301Y | |
| Rifampicin-resistance (18 mutations) | ||
| Internal Control (IC) |
| ANALYTES | Mycobacterium tuberculosis (MTB) | CATALOG NUMBER |
| Fluoroquinolone-resistance (7 mutations) | R-TB7302Y | |
| Injectable drug-resistance (6 mutations) | ||
| Internal Control (IC) |
| ANALYTES | Mycobacterium tuberculosis (MTB) | CATALOG NUMBER |
| Mycobacteria | R-TB7202W | |
| Internal Control (IC) |
Dermatophyte Infections
| ANALYTES | Trichophyton rubrum complex | CATALOG NUMBER |
| T. mentagrophytes complex | Contact Us | |
| T. verrucosum | ||
| T. tonsurans | ||
| Microsporum spp. | ||
| Epidermophyton floccosum | ||
| Candida albicans | ||
| Internal Control (IC) |
Other Infections
| ANALYTES | HSV1 | CATALOG NUMBER |
| HSV2 | Contact Us | |
| VZV | ||
| AdV | ||
| Parvovirus B19(Q) | ||
| Internal Control (IC) | ||
| ———————————- | ||
| BKV(Q) | ||
| JCV | ||
| CMV(Q) | ||
| EBV(Q) | ||
| HHV6 | ||
| HHV8 | ||
| Internal Control (IC) |
| ANALYTES | Borrelia mayonii | CATALOG NUMBER |
| Babesia duncani | R-TD9900W | |
| Borrelia miyamotoi | ||
| Anaplasma phagocytophilum | ||
| Ehrlichia ewingii | ||
| Borrelia burgdoferi sensu stricto | ||
| Babesia microti | ||
| Rickettsia spp. | ||
| Ehrlichia chaffeensis | ||
| Internal Control (IC) |
| ANALYTES | Borrelia mayonii | CATALOG NUMBER |
| Babesia duncani | PN1TBXTDD100CK003 | |
| Borrelia miyamotoi | ||
| Anaplasma phagocytophilum | ||
| Ehrlichia ewingii | ||
| Borrelia burgdoferi sensu stricto | ||
| Babesia microti | ||
| Rickettsia spp. | ||
| Ehrlichia chaffeensis | ||
| Internal Control (IC) |
| ANALYTES | H. Pylori | CATALOG NUMBER |
| A2142G | Contact Us | |
| A2143G | ||
| Internal Control (IC) |
Oncology and Genetics
| ANALYTES | V600E | CATALOG NUMBER |
| Internal Control (IC) | R-BR7100W |
| ANALYTES | b1a1 | CATALOG NUMBER |
| b2a2, | R-BC1110 | |
| b2a3 | ||
| b3a2 | ||
| b3a3 | ||
| c3a2 | ||
| e1a2 | ||
| e1a3 | ||
| Internal Control (IC) |
| ANALYTES | 112Arg | CATALOG NUMBER |
| 158Arg | R-AP1210W | |
| 112Cys | ||
| 158Cys | ||
| Internal Control (IC) |
| ANALYTES | Factor II – G20210A | CATALOG NUMBER |
| Factor V – H1299R | R-TS7600W | |
| Factor V – R506Q | ||
| Factor V – Y1702C | ||
| MTHFR – A1298C | ||
| MTHFR – C677T | ||
| Internal Control (IC) |
| ANALYTES | IVS1-5(G>C) | CATALOG NUMBER |
| CD15(G>A) | Contact Us | |
| CD30(G>C) | ||
| IVS1-1(G>T) | ||
| HbE | ||
| HbS | ||
| FS8/9(+G) | ||
| FS41/42(-TTCT) | ||
| CD15(-T) | ||
| CD16(-C) | ||
| 619bp delection | ||
| 28(A>G) | ||
| 90(C>T) | ||
| Internal Control (IC) |
Nucleic Acid Extraction
The assay was validated with the extraction options listed below.
Manufacturer | Instrument | Extraction Kit | Reaction No. / Catalog No. |
|---|---|---|---|
Seegene | Seegene STARlet (65415-03) | STARMag 96 X 4
Universal Cartridge Kit | 384 reactions
(744300.4.UC384) |
Seegene | Seegene STARlet (65415-03) | STARMag 96 X 4 Viral DNA/RNA 200 C Kit | 384 reactions (EX00013C) |
Hamilton | Microlab STARlet IVD
(173000-075)
| STARMag 96 X 4
Universal Cartridge Kit | 384 reactions (744300.4.UC384) |
Hamilton | Microlab STARlet IVD
(173000-075) | STARMag 96 X 4 Viral
DNA/RNA 200 C Kit | 384 reactions
(EX00013C) |
Seegene | Seegene NIMBUS (67930-03 | STARMag 96 X 4
Universal Cartridge Kit | 384 reactions (744300.4.UC384) |
Seegene | Seegene NIMBUS (67930-03) | STARMag 96 X 4 Viral DNA/RNA 200 C Kit | 384 reactions (EX00013C) |
Hamilton | Microlab NIMBUS IVD
(65415-02) | STARMag 96 X 4
Universal Cartridge Kit | 384 reactions (744300.4.UC384) |
Hamilton | Microlab NIMBUS IVD
(65415-02) | STARMag 96 X 4 Viral
DNA/RNA 200 C Kit | 384 reactions
(EX00013C) |
LG Chem | AdvanSure E3 System (YETS0001EG) | AdvanSure NA EX Kit | 96 reactions Reagent Plate
(RPE0001K01) |
LG Chem | AdvanSure E3 System (YETS0001EG) | AdvanSure NA EX Kit | Proteinase K Tube (RPK0001K01) |
LG Chem | AdvanSure E3 System (YETS0001EG) | AdvanSure NA EX Kit | Strip (E3 System) (YSTP0500KG) |
Amplification and Detection
Manufacturer | Instrument |
|---|---|
Bio-Rad | CFX96 Touch |
The Seegene, Hamilton, LG Chem, extraction reagents/instrumentation can be purchased through Seegene Technologies (CA, US).
Notes
Novaplex assay panels are for Research Use Only.