Research Use Only

Novaplex™ Assays*

High-throughput multiplex testing for identification of unknown pathogens

Seegene’s Novaplex assays provide more information for infections than simplex assays

The Seegene Novaplex Assay panels are Research Use Only (RUO) real-time reverse transcriptase polymerase chain reaction (RT-PCR) tests that maximize test efficiency with excellent performance.

Because Seegene Novaplex assays simultaneously test for multiple targets in reduced running time with minimal labor required. They reduce overall test cost and improve test productivity.

Full panels provide much shorter test times for multiple parasitic, viral or bacterial infections, while reducing labor and reagent/media cost and still providing the highest level of sensitivity.

Seegene’s integrated all-in-one automated platform enable high-throughput testing.

*NOTE: Novaplex assay panels are for Research Use Only.

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Technology

MuDT™

Individual Ct Values for multiple targets MUDT™ is the world’s first Real-time PCR technology that provides individual Ct values for multiple targets.

MuDT™ technology is the novel analytical real-time PCR technology, which enables us to detect multiple targets with individual Ct value in a single channel without melting curve analysis.

TOCE™

The New Paradigm for High multiplex Real-time PCR TOCE™ technology enables confirmation of multiple (five or more) target genetic detection and genetic variation.

TOCE™ Technology is very elegant oligonucleotide chemistry solution for the homogenous high multiplex real-time PCR. TOCE™ technology overcomes the current technical limitations of target-based probe real-time PCR technology and fully exploits the potential of real-time PCR in high multiplex analysis, through unique signal generation & melting temperature analysis by novel components.

DPO™

Novel Oligo platforms of super multiplex PCR.

DPO™ Technology is the innovative multiple targets amplification technology that enhances target specificity and minimizes the non-specific amplification commonly occurred in multiplex PCR. DPO™ technology redefines high multiplex PCR by enabling to detect “many targets in a single tube” and is well-suited for high multiplex real-time PCR technology.

Features and Benefits

Features
Benefits
Proof
MULTIPLEX, REAL-TIME PCR – Detection and differentiation of essential pathogens causing infections in a single reaction
Delivers results faster at a lower cost and more accurately
Comparison of the fast track diagnostics respiratory 21 and Seegene Allplex multiplex polymerase chain reaction assays for the detection of respiratory viruses
MANUFACTURABILITY
Consistent supply
Seegene has delivered hundreds of million tests
ENDOGENOUS INTERNAL CONTROL
Validates correct sampling for self-collected samples
A Novel Multiplex qRT-PCR Assay to Detect SARS-CoV-2 Infection: High Sensitivity and Increased Testing Capacity
SHORT TAT – (4 hours) from extraction to the final results
Delivers results faster to your customers for a faster treatment implementation
POWERFUL PERFORMANCE – PCR with high sensitivity and specificity
Delivers accurate results for better patient care and peace of mind
Performance of Anyplex™ II multiplex real-time PCR for the diagnosis of seven sexually transmitted infections: comparison with currently available methods
USER-FRIENDLY WORKFLOW – Convenient workflow using Seegene’s automated one platform
Implement Seegene’s solution in your laboratory faster with minimum training
MuDT™ – Double up multiplexing capacity in any real-time PCR instrument
Enable any existing Real-time PCR instrument to be more powerful system by doubling up its capability for multiplexing without upgrading the hardware
Single-channel multiplexing without melting curve analysis in real-time PCR
DPO™ PRIMERS – Eliminate non-specific amplification compared to conventional primers that do not eliminate nonspecific amplification
Delivers more accurate results for better pathogen detection
Dual priming oligonucleotide system for the multiplex detection of respiratory viruses and SNP genotyping of CYP2C19 gene
AUTOMATIC DATA ANALYSIS – Automated data interpretation and LIS connectivity with Seegene Viewer.
Easily retrieves results and eliminates interpretation and clerical errors
UDG SYSTEM – Utilization of the UDG system to prevent carry-over contamination
Prevents carryover contamination and false positive results found in PCR for more accurate results
SEMI-QUANTITATIVE RESULTS OR SEMI-QUANTITATION
Patented MuDT technology enables multiple Ct values per fluorescent color channel

Results

Automated data analysis for multiplex real-time PCR

Seegene Viewer is designed to enable users to simply access to automated data analysis for Seegene’s high multiplex real-time PCR assays. The software allows identification and differentiation for both Ct value of multiple targets in a single channel as well as melting curve analysis.

Panels

COVID-19 and Variants

Women's Health

ANALYTESChlamydia trachomatis (CT)CATALOG NUMBER
 Neisseria gonorrhoeae (NG)R-SD9801W
 Mycoplasma genitalium (MG) 
 Mycoplasma hominis (MH) 
 Trichomonas vaginalis (TV) 
 Ureaplasma urealyticum (UU) 
 Ureaplasma parvum (UP) 
 Internal Control (IC) 
ANALYTESHerpes simplex virus type 1 (HSV1)CATALOG NUMBER
 Herpes simplex virus type 2 (HSV2)R-SD9802W
 Cytomegalovirus (CMV)  
 Varicella-zoster virus (VZV) 
 Lymphogranuloma venereum (LGV) 
 Treponema pallidum (TP) 
 Haemophilus ducreyi (HD) 
 Internal Control (IC) 
ANALYTESCandida albicans (CA)Catalog Number
 Candida glabrata (CG)R-SD9803W
 Candida tropicalis (CTp) 
 Candida parapsilosis (CP) 
 Candida krusei (CK) 
 Candida lusitaniae (CL) 
 Candida dubliniensis (CD) 
 Internal Control (IC) 
ANALYTESGardnerella vaginalis (Quantitative) (GV)CATALOG NUMBER
 Atopobium vaginae (Quantitative) (AV)R-SD9804W
 Megasphaera Type 1 (Mega1) 
 Bacterial vaginosis–associated bacteria 2 (BVAB2) 
 Mobiluncus spp. (Mob) 
 Bacteroides fragilis (BF) 
 Lactobacillus spp. (Quantitative) (Lacto) 
 Internal Control (IC) 
ANALYTESEscherichia coli CATALOG NUMBER
 Staphylococcus aureusR-SD9700W
 Enterococcus faecalis 
 Streptococcus agalactiae 
 Streptococcus pyogenes 
 Lactobacillus spp. 
 Lactobacillus reuteri/rhamnosus 
 Internal Control (IC) 
ANALYTESMycoplasma genitalium (MG)CATALOG NUMBER
  A2058GR-SD1017W
  A2058T 
  A2058C 
  A2059G 
  A2059T 
  A2059C 
 Internal Control (IC) 
ANALYTESMycoplasma genitalium (MG)CATALOG NUMBER
  A247CR-SD10170
  G248T 
  G248A 
  G259A 
  G259T 
  G259C 
  A260G 
 Internal Control (IC) 
ANALYTESHuman Papillomavirus 16 (HPV 16)CATALOG NUMBER
 Human Papillomavirus 18 (HPV 18)R-HP7E00W
 Human Papillomavirus 31 (HPV 33) 
 Human Papillomavirus 33 (HPV 33) 
 Human Papillomavirus 35 (HPV 35) 
 Human Papillomavirus 39 (HPV 39) 
 Human Papillomavirus 45 (HPV 45) 
 Human Papillomavirus 51 (HPV 51) 
 Human Papillomavirus 52 (HPV 52) 
 Human Papillomavirus 56 (HPV 56) 
 Human Papillomavirus 58 (HPV 58) 
 Human Papillomavirus 59 (HPV 59) 
 Human Papillomavirus 66 (HPV 66) 
 Human Papillomavirus 68 (HPV 68) 
 Internal Control (IC) 
ANALYTESHuman Papillomavirus 16 (HPV 16)CATALOG NUMBER
 Human Papillomavirus 18 (HPV 18)R-HP7S00W
 Human Papillomavirus 26 (HPV 26) 
 Human Papillomavirus 31 (HPV 33) 
 Human Papillomavirus 33 (HPV 33) 
 Human Papillomavirus 35 (HPV 35) 
 Human Papillomavirus 39 (HPV 39) 19 High-risk HPV genotypes: 
 Human Papillomavirus 45 (HPV 45)16, 18, 26, 31, 33, 35, 39, 45, 
 Human Papillomavirus 51 (HPV 51)51, 52, 53, 56, 58, 59, 66, 68, 69, 73, 82)
 Human Papillomavirus 52 (HPV 52) 9 Low-risk HPV genotypes: 
 Human Papillomavirus 53 (HPV 53)6, 11, 40, 42, 43, 44, 54, 61, 70)
 Human Papillomavirus 56 (HPV 56) 
 Human Papillomavirus 58 (HPV 58) 
 Human Papillomavirus 59 (HPV 59) 
 Human Papillomavirus 66 (HPV 66) 
 Human Papillomavirus 68 (HPV 68) 
 Human Papillomavirus 69 (HPV 69) 
 Human Papillomavirus 73 (HPV 73) 
 Human Papillomavirus 82 (HPV 82) 
 Human Papillomavirus 11 (HPV 11) 
 Human Papillomavirus 40 (HPV 40) 
 Human Papillomavirus 42 (HPV 42) 
 Human Papillomavirus 43 (HPV 43) 
 Human Papillomavirus 44 (HPV 44) 
 Human Papillomavirus 54 (HPV 54) 
 Human Papillomavirus 6 (HPV 6) 
 Human Papillomavirus 61 (HPV 61) 
 Human Papillomavirus 70 (HPV 70) 
 Internal Control (IC) 
ANALYTESChlamydia trachomatis (CT)CATALOG NUMBER
 Neisseria gonorrhoeae (NG)R-SD9400W
 Mycoplasma genitalium (MG) 
 Trichomonas vaginalis (TV) 
 Internal Control (IC) 
ANALYTES Escherichia coli REFERENCE NUMBER
Pseudomonas aeruginosa R-SD10228W
Klebsiella pneumoniae
Klebsiella oxytoca
Klebsiella (Enterobacter) aerogenes
Proteus mirabilis
Proteus vulgaris
Enterobacter cloacae complex
Serratia marcescens
Internal Control (IC)
 ANALYTESEnterococcus faecalisREFERENCE NUMBER
 Enterococcus faeciumR-SD10240W
 Staphylococcus aureus 
 Streptococcus agalactiae 
 Staphylococcus epidermidis 
 Staphylococcus saprophyticus 
 Actinobaculum schaalii 
 Candida albicans 
 Candida others (glabrata, tropicalis, parapsilosis, krusei) 
 Internal Control (IC) 
 ANALYTES Acinetobacter baumannii REFERENCE NUMBER
  Citrobacter freundii R-SD10241W
  Citrobacter koseri  
  Morganella morganii  
  Providencia stuartii  
  Streptococcus anginosus  
  Aerococcus urinae  
  Corynebacterium urealyticum  
  Pantoea agglomerans  
  Internal Control (IC)  

Hospital Acquired Infection

ANALYTES[CPE]CATALOG NUMBER
 Klebsiella pneumoniae carbapenemase (KPC)R-CR9700W
 Verona Integron–encoded Metallo-β-Lactamase (VIM) 
 New Delhi Metallo-beta-lactamase (NDM) 
 Imipenemase (IMP) 
 Oxacillinase-48 (OXA-48) 
 [VRE] 
 vanA 
 vanB 
 [ESBL] 
 CTX-M 
 Internal Control (IC) 
   
MRSASA 
 mecA 
 mecC 
 Internal Control (IC) 

Respiratory Syndromic

ANALYTESInfluenza A virus (Flu A)CATALOG NUMBER
 Influenza B virusR-RP9801W
 Respiratory syncytial virus A  
 Respiratory syncytial virus B  
 Influenza A-H1 (Flu A-H1) 
 Influenza A-H1pdm09 (Flu A-H1pdm09) 
 Influenza A-H3 (Flu A-H3) 
 Internal Control (IC) 
ANALYTESAdenovirus (AdV)CATALOG NUMBER
 Enterovirus (HEV)R-RP9802W
 Parainfluenza virus 1 (PIV 1) 
 Parainfluenza virus 2 (PIV 2) 
 Parainfluenza virus 3 (PIV 3) 
 Parainfluenza virus 4 (PIV 4) 
 Metapneumovirus (MPV)  
 Internal Control (IC) 
ANALYTESBocavirus 1/2/3/4 (HBoV)CATALOG NUMBER
 Rhinovirus (HRV)R-RP9601W
 Coronavirus NL63 (NL63) 
 Coronavirus 229E (229E) 
 Coronavirus OC43 (OC43) 
 Internal Control (IC) 
ANALYTESMycoplasma pneumoniae (MP)CATALOG NUMBER
 Chlamydia pneumoniae (CP)R-PB10175W
 Legionella pneumophila (LP) 
 Haemophilus influenzae (HI) 
 Streptococcus pneumoniae (SP) 
 Bordetella pertussis (BP) 
 Bordetella parapertussis 
 Internal Control (IC) 
ANALYTESAdenovirus (AdV) CATALOG NUMBER
 Influenza A virus (Flu A)R-RV7G01W
 Influenza B virus (Flu B) 
 Parainfluenza virus 1 (PIV 1) 
 Parainfluenza virus 2 (PIV 2) 
 Parainfluenza virus 3 (PIV 3) 
 Parainfluenza virus 4 (PIV 4) 
 Rhinovirus (HRV) 
 Internal Control (IC) 
 ———————————- 
 Respiratory syncytial virus A (RSV A) 
 Respiratory syncytial virus B (RSV B) 
 Bocavirus 1/2/3/4 (HBoV) 
 Metapneumovirus (MPV) 
 Coronavirus 229E (229E) 
 Coronavirus NL63 (NL63) 
 Coronavirus OC43 (OC43) 
 Enterovirus (HEV)  
 Internal Control (IC) 
   
ANALYTESInfluenza A virus (Flu A)CATALOG NUMBER
 Influenza B virus (Flu B)R-RV9750W
 Respiratory syncytial virus (RSV) 
 Metapneumovirus (MPV) 
 Adenovirus (AdV)  
 Parainfluenza virus (PIV) 
 Rhinovirus A/B/C (HRV)  
 Internal Control (IC) 
ANALYTESMycoplasma pneumoniae (MP)CATALOG NUMBER
 Chlamydophila pneumoniae (CP)R-RB7500W
 Legionella pneumophila (LP) 
 Bordetella pertussis (BP) 
 Bordetella parapertussis (BPP) 
 Internal Control (IC) 

Gastrointestinal

ANALYTESNorovirus GI (NoV-GI)CATALOG NUMBER
 Norovirus GII (NoV-GII)R-GI9701W
 Rotavirus (RotV)  
 Adenovirus (AdV)  
 Astrovirus (AstV)  
 Sapovirus (SV) 
 Internal Control (IC) 
ANALYTESCampylobacter spp. (Cam)CATALOG NUMBER
 Clostridium difficile toxin B (CdB)R-GI9703W
 Salmonella spp. (Sal)  
 Shigella spp./EIEC (Sh/EI)  
 Vibrio spp. (Vib) 
 Yersinia enterocolitica (Yer)  
 Aeromonas spp. (Aer) 
 Internal Control (IC) 
ANALYTESHypervirulent Clostridium difficile (CD hyper)CATALOG NUMBER
 Escherichia coli O157 (E. coli O157)R-GI9702W
 STEC (stx1/2)  
 EPEC (eaeA) 
 ETEC (lt/st) 
 EAEC ( aggR ) 
 Internal Control (IC) 
ANALYTESGiardia lamblia (GL)CATALOG NUMBER
 Entamoeba histolytica (EH)R-GI9703W
 Cryptosporidium spp. (CR) 
 Blastocystis hominis (BH) 
 Dientamoeba fragilis (DF) 
 Cyclospora cayetanensis (CC) 
 Internal Control (IC) 
Helminthiasis IMicrosporidiosis  
 Strongyloidiasis 
 Hymenolepiasis 
 Ascariasis 
 Taeniasis 
 Ancylostomiasis 
 Trichuriasis (Trichuris Trichiura) 
 Enterobiasis (Enterobius vermicularis)  
 Ancylostomiasis (Necator Americanus) 
 Internal Control (IC) 

Tuberculosis

ANALYTESMycobacterium tuberculosis (MTB)CATALOG NUMBER
 Isoniazid-resistance (7 mutations) R-TB7301Y
 Rifampicin-resistance (18 mutations) 
 Internal Control (IC) 
ANALYTESMycobacterium tuberculosis (MTB)CATALOG NUMBER
 Fluoroquinolone-resistance  (7 mutations)R-TB7302Y
 Injectable drug-resistance  (6 mutations) 
 Internal Control (IC) 
ANALYTESMycobacterium tuberculosis (MTB)CATALOG NUMBER
 MycobacteriaR-TB7202W
 Internal Control (IC) 

Dermatophyte Infections

ANALYTESTrichophyton rubrum complexCATALOG NUMBER
 T. mentagrophytes complexContact Us
 T. verrucosum 
 T. tonsurans 
 Microsporum spp.  
 Epidermophyton floccosum 
 Candida albicans 
 Internal Control (IC) 

Other Infections

ANALYTESHSV1CATALOG NUMBER
 HSV2Contact Us
 VZV 
 AdV 
 Parvovirus B19(Q) 
 Internal Control (IC) 
 ———————————- 
 BKV(Q) 
 JCV 
 CMV(Q) 
 EBV(Q) 
 HHV6 
 HHV8 
 Internal Control (IC) 
ANALYTESBorrelia mayonii CATALOG NUMBER
 Babesia duncani R-TD9900W
 Borrelia miyamotoi  
 Anaplasma phagocytophilum  
 Ehrlichia ewingii  
 Borrelia burgdoferi sensu stricto  
 Babesia microti 
 Rickettsia spp. 
 Ehrlichia chaffeensis 
 Internal Control (IC) 
ANALYTESBorrelia mayonii CATALOG NUMBER
 Babesia duncani PN1TBXTDD100CK003
 Borrelia miyamotoi  
 Anaplasma phagocytophilum  
 Ehrlichia ewingii  
 Borrelia burgdoferi sensu stricto  
 Babesia microti 
 Rickettsia spp. 
 Ehrlichia chaffeensis 
 Internal Control (IC) 
ANALYTESH. PyloriCATALOG NUMBER
 A2142GContact Us
 A2143G 
 Internal Control (IC) 

Oncology and Genetics

ANALYTESV600ECATALOG NUMBER
 Internal Control (IC)R-BR7100W
ANALYTESb1a1CATALOG NUMBER
 b2a2,R-BC1110
 b2a3 
 b3a2 
 b3a3 
 c3a2 
 e1a2 
 e1a3 
 Internal Control (IC) 
ANALYTES112ArgCATALOG NUMBER
 158ArgR-AP1210W
 112Cys 
 158Cys 
 Internal Control (IC) 
ANALYTESFactor II – G20210ACATALOG NUMBER
 Factor V – H1299RR-TS7600W
 Factor V – R506Q 
 Factor V – Y1702C 
 MTHFR – A1298C 
 MTHFR – C677T 
 Internal Control (IC) 
ANALYTESIVS1-5(G>C)CATALOG NUMBER
 CD15(G>A)Contact  Us
 CD30(G>C) 
 IVS1-1(G>T) 
 HbE 
 HbS 
 FS8/9(+G) 
 FS41/42(-TTCT) 
 CD15(-T) 
 CD16(-C) 
 619bp delection 
 28(A>G) 
 90(C>T) 
 Internal Control (IC) 

Nucleic Acid Extraction

The assay was validated with the extraction options listed below.

Manufacturer
Instrument
Extraction Kit
Reaction No. / Catalog No.
Seegene
Seegene STARlet (65415-03)
STARMag 96 X 4 Universal Cartridge Kit
384 reactions (744300.4.UC384)
Seegene
Seegene STARlet (65415-03)
STARMag 96 X 4 Viral DNA/RNA 200 C Kit
384 reactions (EX00013C)
Hamilton
Microlab STARlet IVD (173000-075)
STARMag 96 X 4 Universal Cartridge Kit
384 reactions (744300.4.UC384)
Hamilton
Microlab STARlet IVD (173000-075)
STARMag 96 X 4 Viral DNA/RNA 200 C Kit
384 reactions (EX00013C)
Seegene
Seegene NIMBUS (67930-03
STARMag 96 X 4 Universal Cartridge Kit
384 reactions (744300.4.UC384)
Seegene
Seegene NIMBUS (67930-03)
STARMag 96 X 4 Viral DNA/RNA 200 C Kit
384 reactions (EX00013C)
Hamilton
Microlab NIMBUS IVD (65415-02)
STARMag 96 X 4 Universal Cartridge Kit
384 reactions (744300.4.UC384)
Hamilton
Microlab NIMBUS IVD (65415-02)
STARMag 96 X 4 Viral DNA/RNA 200 C Kit
384 reactions (EX00013C)
LG Chem
AdvanSure E3 System (YETS0001EG)
AdvanSure NA EX Kit
96 reactions Reagent Plate (RPE0001K01)
LG Chem
AdvanSure E3 System (YETS0001EG)
AdvanSure NA EX Kit
Proteinase K Tube (RPK0001K01)
LG Chem
AdvanSure E3 System (YETS0001EG)
AdvanSure NA EX Kit
Strip (E3 System) (YSTP0500KG)

Amplification and Detection

Manufacturer
Instrument
Bio-Rad
CFX96 Touch

The Seegene, Hamilton, LG Chem, extraction reagents/instrumentation can be purchased through Seegene Technologies (CA, US).

Notes

Novaplex assay panels are for Research Use Only.

Introducing Seegene Mobile Station

High Multiplex RT-PCR Testing for COVID-19 and Other Pathogens